Fig. 7.

Mice with perinatal systemic inflammation show signs of anxiety in the elevated plus maze and open field activity tasks during adolescence. (A) Neonatal male Swiss Webster mice were administered PBS or IL-1β from P1 through P5 and then tested in the elevated plus maze task for exploratory and anxiety behavior at P36. Results are expressed as mean ​± ​SEM; ∗p ​< ​0.05 by unpaired t-test. (B) Total distance travelled was measured using ANY-maze behavioral tracking software. Mice also were tested using open field activity system for exploratory and anxiety behavior at P39. Quantification of time spent in the center zone of the activity chamber during the (C) first 30 ​min and (D) the entire 60 ​min session, (E) total distance travelled and (F) rearing events for control versus IL-1β-treated mice. Results are expressed as mean ​± ​SEM; ∗p ​< ​0.05, ∗∗p ​< ​0.01 by unpaired t-test, n ​= ​11 mice for control group, n ​= ​13 mice for IL-1β group. (G and H) Representative path traces for control versus IL-1β-treated mice during a 60 ​min session. (I) Timeline of treatment and endpoints for these behavioral assessments.