FIG. 1.

Detection of multiplex PCR products with species-specific primers. Random samples of patients’ blood were analyzed. Lanes 1 and 6, blood infected with P. vivax (parasitemias, 1.3 and 0.3%, respectively); lanes 2, 4, 5, and 7, blood infected with P. falciparum (parasitemias, 1.1, 0.8, 0.075, and 0.15%, respectively); lanes 3 and 8, blood from non-malaria-infected patients; lane M, 100-bp marker (Promega). (A) PCR-amplified products were run on a 1% agarose gel in 1× TAE electrophoresis buffer. (B) Paper chromatography hybridization. Oligonucleotide probes specific for P. falciparum (Pf) and P. vivax (Pv) were spotted onto 5-mm-wide nitrocellulose strips. The P. vivax-specific probe was spotted on the top right corner. The P. falciparum-specific probe was spotted on the bottom left corner.