FIG. 3.

PCR monitoring of blood samples from patients undergoing treatment for malaria by using the genus-specific primers L1 and L2. Five microliters of blood spotted on filter paper was assayed in each reaction. Blood samples were obtained daily. For patient A (A; initial parasitemia, 0.35%) and patient B (B; initial parasitemia, 0.5%), PCR products could still be detected after day 5. No PCR product was observed after 4 days for patient C (C; initial parasitemia, 0.3%). Lane M, 100-bp ladder (Promega) used as a molecular size marker.