FIG. 1.

Wild-type and mutated Cbfa motifs in the rat OC gene promoter. The positions and nucleotide numbers of the three Cbfa sites (A, B, and C) relative to the VDRE, the glucocorticoid response element (GRE), a TGFβ-responsive AP-1 site, and two primary transcriptional elements requisite for basal transcription, the OC box and the TATA box, are indicated. The positioned nucleosome and DNase I-hypersensitive sites that are present when the gene is transcribed are also indicated. The Cbfa core recognition sequence at each site is indicated in boldface, with mutant nucleotides (mt) designated below. The lower panel shows the WT oligonucleotide probes, used in gel mobility shift assays, containing the site A, B, and C Cbfa motifs within the context of flanking sequences of the rat OC promoter.