Table 2.
List of secondary antibodies used for immunohistochemistry
| Antigen | Host | Conjugate | Source | Catalog # |
|---|---|---|---|---|
| Anti-mouse IgG | Goat | Alexa 594 | Jackson Immuno Research Labs, West Grove, PA | 115–585-146 |
| Anti-mouse IgG | Goat | Alexa 488 | Jackson Immuno Research Labs | 115–545-166 |
| Anti-rabbit IgG | Goat | Alexa 488 | Jackson Immuno Research Labs | 111–545-144 |
| Anti-rabbit IgG | Goat | Alexa 594 | Jackson Immuno Research Labs | 111–585-144 |
| Anti-rabbit IgG | Donkey | Alexa 488 | Jackson Immuno Research Labs | 711–545-152 |
| Anti-rabbit IgG | Goat | Biotin | Vector Laboratories, Burlingame, CA | BA-1000 |
| Anti-rat IgG | Goat | Alexa 594 | Jackson Immuno Research Labs | 112–585-143 |
| Anti-sheep IgG* | Donkey | Alexa 594 | Invitrogen, Waltham, MA | A-11016 |
Working dilution 1:500, except* diluted 1:400