Table 3.
List of primary and secondary antibodies used for Western immunoblotting
| Antigen | Abbreviation | Host | Dilution | Source | Catalog # (Clone) |
|---|---|---|---|---|---|
| Beta-actin | β-actin | Mouse | 1:10,000 | Sigma-Aldrich, St. Louis, MO | A2228 (AC-74) |
| Cluster of differentiation 230 | CD230 | Mouse | 1:5,000 | BioLegend, San Diego, CA | 808,001 (6D11) |
| Glial fibrillary acidic protein | GFAP | Rabbit | 1:15,000 | Dako/Agilent Technologies Santa Clara, CA | Z0334 |
| Human apolipoprotein E | ApoE | Goat | 1:7,000 | Meridian Life Science Inc., Memphis, TN | K74180B |
| Murine apolipoprotein E | ApoE | Mouse | 1:5,000 | Gift of D.M. Holtzman, Washington University St. Louis, MO [57] | (HJ6.3) |
| Goat IgG, HRP-linked secondary antibody | Donkey | 1:30,000 | Santa Cruz Biotechnology Inc., Dallas, TX | Sc-2020 | |
| Rabbit IgG F(ab’)2 specific, HRP-linked secondary antibody | Donkey | 1:30,000 | GE Healthcare Bio-Science Corp., Pittsburgh, PA | NA9340 | |
| Mouse IgG F(ab’)2 specific, HRP-linked secondary antibody | Donkey | 1:30,000 | GE Healthcare Bio-Science Corp., Pittsburgh, PA | NA9310 |