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. 2021 Sep 26;21:1059. doi: 10.1186/s12885-021-08774-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — EMMPRIN expression in osteosarcoma by reverse transcription-PCR and Western blot. a Reverse transcription-PCR products of total RNA in tumor-derived osteosarcoma cells from 6 patients. b Comparison of grayscale ratio of EMMPRIN/β-actin in tumor-derived osteosarcoma cells and MG63. *, p < 0.05 compared with MG63. c EMMPRIN mRNA expression after transfection of SaOS-2 cell by EMMPRIN-targeting siRNA. Data shown are representative images of individual cell lines from three separate experiments. d Western blot analysis of EMMPRIN protein expressions. β-actin was used as loading control