Sizes (in base pairs) and approximate locations of FAFLP assay-predicted fragments in the E. coli K-12 MG1655 genome obtained with primer set MseI+TA–EcoRI+0. The genes that the fragments are predicted to have been amplified from are also indicated. θ, an uncharacterized locus in E. coli bearing sequence similarity to characterized loci in other bacteria; ϕ, no matches were found, indicating a noncoding region; ∗, hypothetical, as yet uncharacterized genes.