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. 2021 Aug 5;10(16):e020554. doi: 10.1161/JAHA.120.020554

Figure 1. Angiotensin II (Ang II)–induced interleukin 11 (IL‐11) expression in adventitia.

Figure 1

A, Representative immunofluorescence staining and quantitative analysis of IL‐11 (red), α‐smooth muscle actin (ACTA2) (green) in thoracic aorta. The 4′,6‐diamidino‐2‐phenylindole was used to stain the nucleus in blue. Bar=50 µm. B, IL‐11 mRNA was stained with fluorescence by situ hybridization assay. Bar=50 µm. C, The mRNA level of IL‐11 (1.00‐ and 12.47‐fold) in thoracic aorta of wild‐type mice with or without Ang II infusion was quantified by quantitative reverse transcription–polymerase chain reaction (RT‐qPCR). D, The media (M) and adventitia (A) were treated with Ang II (10−6 mol/L) for 4 hours. The mRNA level of IL‐11 was quantified by RT‐qPCR (1.00‐, 8.23‐, 2.18‐, and 15.4‐fold). I indicates intima. M indicates media; and A indicates adventitia. **P<0.01, ***P< 0.001.