Figure 3. Assessment of expression of selected genes in human adult and aging myocardium.
Depicted are mRNA expression levels of selected genes associated with ROS metabolism in human adult (age 18–65 years, n=7) and aging (age >65 years, n=10) myocardium. A, Scatter plot examining age‐dependent correlation of age and mRNA gene expression reveals significant inverse correlation in age vs NDUFA6 (R 2=0.41), with total mRNA expression values from adult and elderly patients. Bars highlighting distribution of individual gene expression values along with mean±standard deviation values for each age group (inset). B, Inverse correlation in age vs NOX2 mRNA expression (R 2=0.005). Total mRNA expression shown in inset. C, TXN2 (R 2=0.304) shows significant inverse correlation with age, with significant total mRNA downregulation in elderly patients compared to adults (inset). D, No significant correlation was seen in age vs TXNRD3 (R 2=0.08); however, an upward trend is observed. Data were analyzed by regression analysis of the sample population (n=17), *P<0.05.