FIG. 5.

Hydrolysis of ATP is not required for dissociation of p53-DNA complexes. (A) Time course analysis of p53 release from sequence-specific DNA. Samples were incubated with ATP, ATP-γ-S, or ADP. Controls involve incubations without nucleotide addition. After the given times (5, 10, 20, 30, and 60 min), fractions of released and DNA-bound p53 were quantitated by scintillation counting. (B) p53-DNA complexes were initially stabilized with ADP and subsequently incubated with nucleotides as described above. For both experiments, the data represent an average of four independent experiments. “beads,” control incubation of beads, to which p53 was bound without DNA and subsequently washed (see Fig. 1A and Materials and Methods) to remove unbound protein before the incubation.