Figure 3.

Detecting cell growth by colony formation assay after docetaxel, LBH-589, and pralatrexate treatments. U-Hth83 (A) and K2 (B) cells (100 cells/well) were plated in 6-well plates in triplicates, and only 1 well was shown here as an example. Docetaxel, LBH-589, and pralatrexate at different concentrations were added to cells 24 h later for 72 h. After 72-h treatment, agents were removed and fresh media were added to cells. Cells were then incubated for up to 7 days without disturbance to allow colonies to grow. To visualize colonies, cells were stained with 0.05% crystal violet in 10% formalin. Controls were cells without agent treatment. Colony numbers were converted to surviving fractions by Prism after transforming colony numbers with log.