TABLE 2.
Overview of experimental analyses performed with viruses carrying different HA subtypes in this studya
| Virus | Mouse lung cells |
Calu-3 cells |
HBEC |
Duck TMPRSS2 |
||||
|---|---|---|---|---|---|---|---|---|
| Virus growth | WB | Virus growth | WB | Virus growth | WB | Virus growth | WB | |
| H1-SC35M | ✓ | ND | ND | ND | ND | ND | ✓ | ND |
| H2-SC35M | ✓ | ND | ✓ | ND | ✓ | ND | ✓ | ND |
| H3-SC35M | ✓ | ND | ✓ | ND | ND | ND | ND | ND |
| H4-SC35M | ✓ | ✓ | ✓ | ✓ | ✓ | ND | ✓ | ✓ |
| H5N1-PR8 | ✓ | ND | ✓ | ✓ | ✓ | ✓ | ✓ | ND |
| H6-SC35M | ✓ | ND | ✓ | ND | ND | ND | ✓ | ND |
| H7-PR8 | NDb | NDb | NDb | NDb | NDb | NDb | ✓ | ✓ |
| H8-SC35M | ✓ | ND | ✓ | ND | ND | ND | ✓ | ND |
| H9-SC35M | ✓ | ND | ND | ND | ND | ND | ✓ | ND |
| H10-SC35M | ✓ | ND | ✓ | ND | ✓ | ND | ✓ | ND |
| H11-SC35M | ✓ | ND | ✓ | ND | ND | ND | ✓ | ND |
| H14-SC35M | ✓ | ND | ✓ | ND | ND | ND | ✓ | ND |
| H15-SC35M | ✓ | ND | ✓ | ND | ND | ND | ✓ | ND |
| H16-SC35M | ✓ | ND | ✓ | ND | ✓ | ND | ✓ | ND |
| H3N2/Victoria | ✓ | ND | ND | ND | ND | ND | ND | ND |
| H3N2/HongKong | ND | ND | ND | ND | ND | ND | ✓ | ✓ |
| H9N2/Shantou | ND | ND | ✓ | ✓ | ✓ | ND | ND | ND |
| H9N2/Wisconsin | ND | ND | ✓ | ✓ | ✓ | ND | ND | ND |
| IBV | ✓ | ✓ | ✓ | NDb | ✓ | NDb | ND | ND |
a
HA reassortants or wild-type viruses were analyzed for multicycle replication (virus growth) and HA cleavage by Western blotting (WB) in the indicated cell cultures and in MDCK cells transiently expressing duck TMPRSS2. WB analysis of HA cleavage was limited to HA subtypes H3, H4, H5, H7, H9, and IBV HA due to the availability of HA-specific antibodies. ✓, analysis performed; ND, not determined.
b
Determined in Limburg et al. (22).