FIG 2.

The flexible PHP.B peptide presents similarly in AAV9 and AAV1 contexts; peptide insertion has a minimal impact on capsid structure. (A and B) Electron density maps contoured at 1σ (gray mesh) and fitted model residues for HVR-VIII of the AAV9-PHP.B capsid (A, green) and AAV1-PHP.B capsid (B, salmon). Red text indicates residues from the PHP.B peptide insertion, and black text indicates residues from the parental capsid. (C and D) Solved structures of AAV9-PHP.B (C) or AAV1-PHP.B (D) superimposed onto previously reported structures for AAV9 (orange) or AAV1 (purple). All figures were rendered using PyMol.