FIG 4.

Kinetics of induction and stability of IFITM1, IFITM2, and IFITM3 expression in A549 cell lines. (A and B) FLAG expression in IFITM1i, IFITM2i, and IFITM3i A549 cell lines at different times (0 to 24 hours) post-DOX induction (IFITM induction) (A) and different times after withdrawal of DOX, where cells were DOX induced for 24 hours and then washed and cultured in DOX-free media (0 to 48 hours) (IFITM stability) (B). For both (A) and (B), representative histograms from 2 independent experiments are displayed in (i) and the geometric mean fluorescent intensity (gMFI) of each histogram is graphed in (ii). The dotted-line in (Bii) represents half-maximal IFITM expression.