FIG 4.
P123 components of viruses belonging to the SFV complex are compatible with heterologous nsP4 proteins. (A to E) HEK293T cells were cotransfected with HSPolI-FG-SINV and (A) CMV-P123-CHIKV, (B) CMV-P123-ONNV, (C) CMV-P123-SFV, (D) CMV-P123-RRV, or (E) CMV-P123-MAYV. In each transformation, one plasmid, expressing nsP4 from a virus shown on the x axes, was added in an equimolar amount to the indicated CMV-P123 plasmid. Transfected cells were incubated at 37°C and lysed for 18 h p.t.; in addition, cells transfected with CMV-ubi-nsP4-EILV were incubated at 28°C and lysed for 48 h p.t. The data represent the activity of Fluc and Gluc from CMV-ubi-nsP4-transfected cells normalized to the paired CMV-P1234GAA control cells. Values obtained for P1234GAA controls were taken as 1. The means ± SD from three independent experiments are shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; n.s., not significant (Student’s unpaired t test).