TABLE 2.
icDNA | Transfections |
||||||||
---|---|---|---|---|---|---|---|---|---|
37°C |
28°C |
Infections with p0 stock |
|||||||
ICA | Virus collected |
Virus collected |
37°C |
28°C |
|||||
PFU/μg | h p.t. | CPE | h p.t. | CPE | h p.i. | CPE | h p.i. | CPE | |
SFV6 | 250,000 | 24 | Cell death | 48 | Cell death | 24 | Cell death | 48 | Cell death |
SFV-SFV4 | 200,000 | 24 | Cell death | 48 | Cell death | 24 | Cell death | 48 | Cell death |
SFV-CHIKV4 | <2 | 72 | Strong | 96 | Very mildb | 24 | Cell death | 72 | Very mildb |
SFV-RRV4 | <2 | 72 | Mild | 96 | Very mildb | 24 | Cell death | 72 | Very mildb |
SFV-SINV4 | <2 | 72 | Strong | 96 | Very mildb | 24 | Cell death | 72 | Very mildb |
SFV-BFV4 | <2 | 72 | Medium | 96 | Very mildb | 24 | Cell death | 72 | Very mildb |
SINV (Toto1101) | 80,000 | 24 | Cell death | 48 | Cell death | 24 | Cell death | 48 | Cell death |
SINV-SINV4 | 80,000 | 24 | Cell death | 48 | Cell death | 24 | Cell death | 48 | Cell death |
SINV-BFV4 | <2 | 72 | Not observed | 48 | Strong | 72 | Not observed | 48 | Cell death |
SINV-EILV4 | <2 | 72 | Not observed | 72 | Strong | 72 | Not observed | 48 | Cell death |
SINV-VEEV4 | <2 | 72 | Not observed | 96 | Mild | 72 | Not observedc | 48 | Cell death |
SINV-CHIKV4 | <2 | 72 | Not observed | 72 | Strong | 72 | Not observed | 48 | Cell death |
SINV-ONNV4 | <2 | 72 | Not observed | 48 | Strong | 72 | Not observed | 48 | Cell death |
SINV-MAYV4 | <2 | 72 | Not observed | 48 | Strong | 72 | Not observed | 48 | Cell death |
SINV-RRV4 | <2 | 72 | Not observed | 96 | Medium | 72 | Not observed | 48 | Cell death |
SINV-SFV4 | <2 | 72 | Not observed | 96 | Not observed | 72 | Not observed | 48 | Cell death |
Data from one experiment out of two are shown.
Signs of CPE were observed 24 h before virus stock was collected, but CPE did not progress and no cell death was observed.
In one experiment, a slight change of color of growth medium was observed.