Figure 8.

Elevated IgG2a titers induced by adjuvanting with PCEP. Mice were immunized i.m. with 2.0 µg RG1-VLPs alone or adjuvanted with 50 µg alum (Alhydrogel), 50 or 15 µg PCEP, or Gardasil-9 on days 0, 14, 28 and peripheral blood sera samples were derived on day 42. (A-B) Sera samples were tested for HPV16-L1- and HPV16-L2 RG1-specific IgG2a via ELISA. Data are reported as geometric means ± 95% CI. Statistical comparisons are between VLPs alone and adjuvanted groups (lower tier), or between alum and PCEP groups (upper tier) and were generated using one-way ANOVA nonparametric analysis with the Kruskal-Wallis multiple comparisons test. ns, not significant (p > .05); **, p < .01; ***, p < .001; ****, p < .0001