Figure 5. Wnt inhibitor increases fatty acid oxidation in podocytes.

A, Immunofluorescence analysis and quantification of collagen I (fluorescein isothiocyanate–labeled collagen 1 and DAPI blue nuclei) in diabetic control and GR^PKO mice in the untreated, Wnti‐treated and etomoxir‐treated conditions. B and C, Quantitative polymerase chain reaction gene expression analysis of β‐catenin, axin2, cpt1a, and pgc1α, in podocytes isolated from mice subjected to the treatment conditions (D). Radiolabeled [C¹⁴]palmitate uptake analysis in isolated podocytes from indicated groups. CPM of each sample was counted. E, Radiolabeled [¹⁴C]palmitate oxidation and [¹⁴CO₂] release were measured in indicated groups. Data mean±SEM. *P<0.05; **P<0.01; ***P<0.001. One‐way ANOVA with Tukey's post hoc test was used. CPM indicates counts per minute.