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. 2021 Aug 26;162(12):bqab179. doi: 10.1210/endocr/bqab179

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Published by Oxford University Press on behalf of the Endocrine Society 2021.

This work is written by (a) US Government employee(s) and is in the public domain in the US.

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Figure 5. — Dio2+ osteoblast lineage cells in Dio2CreERt2;Ai6 mice. A, Dio2+ cells (pale blue) stained with Sp7 osteogenic marker (magenta) in the immature bony capsule. Lower case letters (b-g) indicate approximate areas of magnified views in panels B-G. In all panels, nuclei are stained dark blue (DAPI). In all panels, analysis was at P10, after tamoxifen administration at P3/P4. Mid-basal turn sections. B, C, Examples of Dio2+ osteoblasts (arrowheads) stained with Sp7 in the otic capsule (B) and bony septal division (C). D, E, Dio2+ osteoblasts stained with early-stage factor Runx2 in the otic capsule (D) and near a vessel canal (#) in the modiolus (E). F, G, Dio2+ osteoblasts outlined by Tmem119 staining in the lateral otic capsule (F) and cells lining the bone near a vessel canal (#) at the base of the cochlear turn (G). Abbreviations: SM, scala media; ST, scala tympani; SV, scala vestibuli.