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. 2021 Sep 13;12:738619. doi: 10.3389/fpls.2021.738619

Figure 1.

Figure 1

Schematic representation of plant expression constructs pTRAkc-EDIII-SC (A) and pEAQ-ST-AP205 (B). The SpyCatcher (SC) peptide (red) is fused to the 3′ end of the West Nile virus–envelope protein domain III (WNV-EDIII) gene (blue) by a flexible linker; (GGGGS)2 (white box). The leader peptide derived from heavy chain (LPH) (murine mAB24 heavy chain) signal peptide allows for the translocation of WNV-EDIII-SC to the endoplasmic reticulum. The SpyTag (ST) peptide (orange) is fused to the 5′ end of AP205 coat protein (CP) gene (purple) by a flexible linker; (GGGGS)2 (white box).