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. 2021 Sep 13;12:738619. doi: 10.3389/fpls.2021.738619

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Copyright © 2021 Stander, Chabeda, Rybicki and Meyers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression and purification of plant-produced WNV-EDIII-SC (25 kDa). (A) Immunoblot of crude leaf extracts (4 dpi) of WNV-EDIII-SC co-infiltrated with either CNX or CRT. Lanes were loaded with 25 μg TSP each for direct comparison and WNV-EDIII-SC protein was detected with in-house-produced anti-WNV-EDIII serum. (B) Coomassie blue-stained SDS-PAGE of eluted fractions of WNV-EDIII-SC following pH precipitation and nickel-affinity chromatography. pTRAkc: pTRAkc-ERH empty plant-expression vector. CNX, calnexin; CRT, calreticulin; F, fraction.