Figure 5.

Indirect ELISAs of mouse sera using Escherichia coli produced WNV-EDIII as antigen. (A) Antibody responses before and after immunisation of vaccine groups. Pooled mouse sera (n = 6 per group) for each vaccine group, pre-immunised and immunised bleed was diluted to 1:1,000 and evaluated for the presence of anti-WNV-EDIII antibodies. Bars represent the mean ± SEM from three independent experiments. The y-axis shows the log10 absorbance readings at 405 nm. (B) Comparison of mouse antibody responses post-immunisation of individual mouse sera (n = 6) for each vaccine group as tested at the dilution 1:1,000. Shown are the individual absorbance readings and group means (log10 absorbance at 405 nm). The student's t-test was used to determine the significance levels (p-values are indicated between groups). (C) A 4-fold titration of mouse antiserum (n = 6) from 1:500 to 1:128,000 produced against pTRAkc-ERH negative control, AP205 VLPs displaying WNV-EDIII and soluble WNV-EDIII vaccine candidates. The mean ± SEM of three independent experiments with technical triplicates for each group is presented. NS, * and ** indicate p values > 0.05, < 0.05, and < 0.01, respectively.