KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-KRAS | Sigma-Aldrich | Cat# SAB1404011 |
| Rabbit monoclonal anti-phospho-ERK | Cell Signaling Technology | Cat# 4370S |
| Rabbit monoclonal anti-ERK | Cell Signaling Technology | Cat# 4695S |
| Mouse monoclonal anti-alpha-Tubulin | Cell Signaling Technology | Cat# 3873S |
| Chemicals, Peptides, and Recombinant Proteins | ||
| GeneArt® site-directed mutagenesis system for Human KRAS G12C | Life Technologies | Cat# A13282 |
| KRAS G12C and WT | Westover lab | N/A |
| Deposited Data | ||
| KRAS G12C/compound 1 structure | Protein databank | PDB: 5V71 |
| Experimental Models: Cell Lines | ||
| Human: H358 (Male) | Pasi Janne Lab | ATCC-CRL-5807 |
| Human: A549 (Male) | Pasi Janne Lab | ATCC-CCL-185 |
| Human: H441 (Male) | Pasi Janne Lab | ATCC-HTB-174 |
| Human: HCT116 (Male) | Pasi Janne Lab | ATCC-CCL-247 |
| Recombinant DNA | ||
| Plasmid pcDNA2.0_KRAS_WT | Westover lab | N/A |
| Plasmid pcDNA2.0_KRAS_G12C | Westover lab | N/A |
| Software and Algorithms | ||
| GraphPad Prism | Graphpad Software Inc | https://www.graphpad.com/scientific-software/prism/ |
| DynamX 3.0 | Waters | http://www.waters.com/waters/ |
| HKL2000/3000 | HKL Research, Inc. | http://www.hkl-xray.com/hkl-3000 |
| Phenix | https://www.phenix-online.org/ | |
| Coot | https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ | |
| Pymol | The PyMOL Molecular Graphics System, Version 1.5.0.4 | Schrödinger, LLC |
| ChemDraw Professional 15.0 | PerkinElmer | http://www.cambridgesoft.com/ |