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. 2021 Sep 27;16(9):e0257984. doi: 10.1371/journal.pone.0257984

Fig 5. Bergamottin causes apoptosis in LNCaP and MDAPCa2b cells.

Fig 5

(A & B) LNCaP and MDAPCa2b cells were treated with bergamottin (10μM) or DMSO (control) for 4 days. Cells were then fixed and stained for tunnel assay reagents as described in methods. Experiment was repeated three times and a representative experiment is shown. Size bar 50μm. DAPI was used to stain the nucleus. (C) Western blot analysis showing cleaved PARP after bergamottin treatment (5 and 10 μM for 96 hrs. and 20 μM for 48 hrs.), GAPDH was used as an internal control. (D) Western blot showing PARP cleavage after CYP3A5 siRNA and NT (non-target) siRNA treatment (96 hrs.).