Fig 3. Inflammatory cell response in the adipose tissue.

(A) Immunohistochemistry reveals a large number of T. brucei parasites (anti-VSG antibody) accompanied by marked infiltration by macrophages (anti-F4/80 antibody) and moderate infiltration by T cells (anti-CD3 antibody), at a later stage post-infection (day 26). (n = 4–6 per time-point); DAB counterstained with hematoxylin, original magnification 40x (Scale bar, 50μm). (B). Representative electron micrograph of the perirenal adipose tissue of the mouse, 26 days post-infection, showing various extracellular tangential and cross-sectional profiles of trypanosomes (arrowhead) and also intracellular parasites, in the cytoplasm of a phagocyte [most probably a macrophage (inside dashed line)]. Asterisk, vessel; ec, endothelial cell; n, nucleus, f, flagellum. B-1, B-2. Insets of the phagocytized trypanosomes, with well-defined nucleus (black arrowhead) and pseudopodia (white arrowhead), consisting of multiple membranous whorls extended by the phagocyte around the parasite.