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. Author manuscript; available in PMC: 2022 Jul 6.
Published in final edited form as: Biochemistry. 2021 Jun 24;60(26):2130–2151. doi: 10.1021/acs.biochem.1c00246

Figure 6. Average CαRMSF for CheY variants during simulation.

Figure 6.

Per-residue CαRMSF was calculated across production replicates to quantify atomic fluctuations during the simulations. Solid lines indicate ensemble averages. Colored shaded regions represent standard deviations of the mean (one frame = 20 ps; blue = CheY A113P, red = wild-type CheY). Grey shaded areas indicate regions of statistically significant differences detected between the two CheY variants (two-tailed t-test, p < 0.01, minimum mean difference > 0.15 Å).