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. 2021 Sep 1;10:e68578. doi: 10.7554/eLife.68578

Figure 6. The NB-2D11 blocks the interaction of LB2 domains.

Figure 6.

(A) Structure of the inactive CaSR protomer (surface representation, cyan) with NB-2D11 (ribbon diagram, orange) from front view. (B) The NB-2D11 (orange) binds the left lateral of the LB2 (cyan) from the front view of the protomer. (C) NB-2D11 binds the LB2 domain through a series of polar interactions through CDR1 and CDR3 of the nanobody and the Helix F and Strand I of the CaSR. (D) Superposition of NB-2D11 (orange) binding inactive conformations (cyan) and active (purple) conformations based on the LB2 domain of VFT module, showing the whole NB-2D11 in the inactive state crashes with the LB2 domain of another VFT module in the active state.