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. 2021 Sep 23;14:6009–6019. doi: 10.2147/IJGM.S330389

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© 2021 Yu et al.

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The generation of induced Tregs. (A) Freshly isolated CD4⁺CD25⁻ T cells from PEMCs of an NSCLC patient by magnetic bead sorting. (B and C) CD4⁺CD25⁺Foxp3⁺ Tregs were produced by cytokine stimulation. (D and E) Helios expression in induced Tregs and non-Tregs, respectively.