Skip to main content
. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Nat Immunol. 2021 Apr 15;22(5):627–638. doi: 10.1038/s41590-021-00909-1

Fig. 1 |. Cytokine levels and phosphorylation of STATs in NK cells in spleen and blood peak early during MCMV infection.

Fig. 1 |

Spleens and blood were collected at days 0, 0.5, 1, 2, 3, 4, 5, 7 and 14 after MCMV infection. Spleens were lysed for protein extracts and serum was collected from blood samples for cytokine measurement. NK cells from spleens and blood were stained for p-STAT1 (Ser), p-STAT4 (Tyr) and p-STAT5 (Tyr). a, Graphs show cytokine levels (pg ml−1) of IFN-α, IL-12p70, IL-18, IL-2 and IL-15 in the spleen over time, with log10 scale on the y axis. Lines connect at medians of each time point. *P = 0.011–0.045, **P = 0.0073–0.0079 from two-sided Mann–Whitney–Wilcoxon test as compared to day 0. b, Same as a, in blood serum. c, Histograms (left) and bar plots (right) depict mean ±s.d. of phosphorylated STAT levels on NK cells (CD3ε−TCRβ−NK1.1+) in the spleen at indicated time points after infection. P values are calculated from two-sided Welch’s t-test as compared to day 0. d, Same as c, in blood serum. MFI, mean fluorescence intensity. n = 5 (a,b) and n = 3–5 (c,d).