Fig. 3 |. The ability of phase-separated Dcp1/Dcp2_ext to decap RNA is modulated by Edc3.

a, Synthesis scheme for two-color fluorescent 38mer RNA capped with fluorescein m⁷G cap (FAM-m⁷GDP) and labeled at the 3’ end with Cy5-adenosine. b, c, Addition of dual-labeled RNA to Dcp1/Dcp2_ext (60 μM total protein concentration) droplets shows minimal signal loss in both FAM-m⁷GDP and Cy5-RNA over twenty minutes. d, e, Time-dependent loss of FAM-m⁷GDP and Cy5-RNA signal from droplets formed by 5 μM Dcp1/Dcp2_ext and 80 μM Edc3. f, Addition of substoichiometric Edc3 to preformed Dcp1/Dcp2_ext droplets (60 μM total Dcp1/Dcp2_ext concentration) results in dose-dependent loss of FAM-m⁷GDP signal from droplets. m⁷GDP intensity is normalized to initial fluorescence signal. g, Edc3 activates Dcp1/Dcp2_ext up to 90-fold in condensates. Representative micrographs and data are from twenty droplets examined over two independent experiments with similar results. Data are presented as mean ± s.e.m.