Extended Data Fig. 3. Edc3 alters Dcp1/Dcp2_ext enrichment and RNA mobility in liquid droplets.

a, Superstoichiometric Edc3 enriches Dcp1/Dcp2_ext in droplets and causes causes droplets to be more sensitive to presence of RNA. Images below graph correspond to representative Cy5-labelled Dcp1/Dcp2_ext micrographs and data presented are mean ± s.e.m. for five independent experiments with similar results. b, Enrichment of FAM-29mer RNA in droplets of varying Dcp1/Dcp2_ext and Edc3 concentrations. Representative micrographs and data presented are mean ± s.e.m. for ten independent experiments with similar results. c, Edc3 increases the mobile fraction of RNA in droplets. Dcp1/Dcp2_ext concentration is 40 μM in absence and 5 μM in presence of 80 μM Edc3. Data presented are mean ± s.e.m. for twenty recovery profiles collected over two independent experiments. Error bars are not depicted when smaller than the data point.