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. 2021 Sep 28;27(36):6093–6109. doi: 10.3748/wjg.v27.i36.6093

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©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.

This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/

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Representative agarose gel picture of polymerase chain reaction amplification (A) and restriction fragment length polymorphism using BstN1 (B) for KRAS codon 12. The arrow represents the 157 bp amplicon and M denotes the DNA marker (50 bp). Lane M represents a DNA marker (50 bp). Lanes 2, 4, 7, 8, and 10 represent the mutant band (undigested) of 157 bp. Lane 1, 3, 6, and 9 represent the wild band (digested) of 128 bp. U represents the undigested band used as mutant control.