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TABLE 3.

Detailed analysis of reactivity patterns of samples with discrepant results by screening and confirmatory assays

Sample no. ELISA ratioab
WB2.3 reactivity pattern WB resultb
INNO-LIA reactivity pattern INNO-LIA result PCR resultc
First ELISA
Second ELISA
Org Emb Mur Orth WB2.3 WB2.4
255 1.50 1.97 0.27 1.07 p19, gp21, p24, p26, p28, p53 Ind NT p19, p24, gp46, gp46-1 HTLV-1 Neg
348 0.61 0.28 0.26 0.51 p24 Ind NT p19, p24, gp46, gp46-1 HTLV-I Neg
404 0.85 0.81 0.34 0.40 p19, gp21, p26, p28 Ind NT p19, gp46, gp46-1 HTLV-I tax 
445 3.06 0.04 0.29 0.28 p24, p28 Ind NT p24, pg46, gp46-1 HTLV-I Neg
461 1.55 0.16 0.27 0.96 rgp21, p24 Pos Pos p24, gp46, gp46-1 HTLV-I Neg
1157 0.76 1.26 0.72 0.73 p19, gp21, p26, p28, p53 Ind Ind p19, gp46, gp46-1 HTLV-I Neg
1270 1.05 2.18 0.28 0.75 p19, gp21, p26, p28, p32, p36 Ind NT p19, gp46, p19-1, gp46-1 HTLV-I Neg
314 1.43 1.64 1.53 NT p19, gp21, p28 Ind NT p19, gp46, gp46-2 HTLV-II tax
343 1.95 0.89 0.31 0.26 p24 Ind Ind p24, gp46, gp46-2 HTLV-I Neg
256 2.87 2.01 0.29 0.06 p24, p53 Ind Ind p19, p24, gp21 Pos Neg
274 1.42 1.55 0.34 0.88 p19, gp21, p26, p28, p32, p36, p53 Ind NT p19, gp46 Pos Neg
297 2.45 1.28 1.00 0.71 rgp21, p24 Pos Ind p24, gp46, gp21 Pos Neg
419 0.85 1.59 0.26 1.22 p19, gp21, p26, p28, p32, p36, p53 Ind Ind p19, gp46 Pos NT
426 2.03 3.17 0.30 0.64 p19, gp21, p26, p28, p32, p36, p53 Ind Ind p19, gp46 Pos Neg
432 2.98 1.99 0.31 1.97 p19, gp21, p26, p28, p32, p36, gp46, p53 Ind Ind p19, gp21 Pos Neg
441 1.22 0.55 0.28 0.57 p19, gp21, p28 Ind NT p19, p24, gp46 Pos Neg
460 1.58 0.36 10.24 0.28 rgp21, p19, gp21 Pos Pos p19, gp21 Pos Neg
1091 1.23 1.64 0.24 0.47 rgp21, p19, gp21 Pos Ind p19, gp21 Pos Neg
1100 1.66 2.39 0.32 0.44 rgp21, p19, gp21, p26, p28, p36 Pos Ind p19, gp46 Pos Neg
1164 0.49 0.87 0.89 0.53 p19, gp21, p24, p26, p28, p53 Ind Pos p19, gp21 Pos Neg
1175 1.71 2.34 0.27 0.21 p19, gp21 Ind Pos p19, gp46 Pos Neg
a

ELISA ratios (signal/cutoff) were calculated as described by each manufacturer. For the kits from Organon (Org) and Embrabio (Emb), the results are average ratios of two determinations. For the kits from Murex (Mur) and Ortho (Orth), the results are ratios of a single determination. 

b

NT, not tested; Ind, indeterminate; Pos, positive. 

c

Neg, negative; tax, positive only for tax PCR.