Fig. 5.

Zinc and PMA increases cell death during the respiratory burst

A) Dotplots of the two PLB985 cell lines: SBW cells (don not express NOX2) and SgpslBW cells (do express a functional NADPH oxidase). Gates are set according to the expression of BFP (blue fluorescence protein). Cell death is assessed via the uptake of membrane impermeable PI. Increasing concentrations of zinc lead to more dead cells. B) Summary of three individual experiments with 80 min incubation at 37 °C. Data is depicted as Mean + SD. 120 nM PMA increases cell death, which is augmented by rising concentrations of zinc. 300 μM of zinc has an impact on cell viability with and without PMA. Abbreviations: BFP + cells fluorescence blue, PI + cells have taken up PI.