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. 2021 Sep 27;11:19116. doi: 10.1038/s41598-021-98771-6

Figure 1.

Figure 1

TMZ prevents palmitate (PA)-induced muscle cell damage. Con control group without palmitate treatment, PA skeletal muscle cells treated with palmitate, TMZ skeletal muscle cells treated with trimetazidine. (a, b) MyHC-II immunofluorescence staining was performed to observe the myotube cell morphology and myotube diameter with a 100 μm scale. (c, d) MyHC-II, MuRF1, and Atrogin1 protein expression were detected by Western blot. *, ** represented p < 0.05, p < 0.01 in comparison with the Con group, respectively. #, ## represented p < 0.05, p < 0.01 in comparison with the PA  group, respectively.