Table 1.

Phenotypes of SUN1 variants.

SUN1	Restriction of HIV-1a	Binding to HIV-1 CA-NC complexesb	Localizationc
WT-FLAG	++	+	PS
∆(1–20)-FLAG	++	+	PS
∆(1–40)-FLAG	+	+	PS + NP
∆(1–60)-FLAG	+	+	PS + NP
∆(1–80)-FLAG	+	+	PS + NP
∆(1–100)-FLAG	−	−	PS + NP

^aRestriction was measured by infecting cells expressing the indicated SUN1 variant with HIV-1-GFP. After 48 h, the percentage of GFP-positive cells (infected cells) was determined by flow cytometry. “++”: indicates strong restriction, “+” indicates moderate restriction, “−”: indicates absence of restriction.

^bBinding to the HIV-1 capsid complexes was determined for each SUN1 variant as described in “Methods”. “+”: indicates binding, “−”: indicates no binding.

^cWild type and mutant SUN1-FLAG proteins were assayed to determine localization. “PS” indicates Perinuclear staining, “NP” indicates Non-Perinuclear localization. “PS + NP” indicates both perinuclear and non-peri-nuclear staining is observed.