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. 2021 Sep 27;4:1133. doi: 10.1038/s42003-021-02662-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic of the developmental cycle of follicles during mouse folliculogenesis. A follicle consists of the oocyte (pink) with its nucleus (blue), surrounded by the somatic cells (orange) and theca cells (gray). The oocyte grows in size during transition to secondary follicle stage, followed by the emergence of fluid-filled lumen (blue) in the antral follicle stage. The oocyte is eventually released during ovulation, and upon fertilization undergoes embryo development. The interstitial tissues of the ovaries comprise of stromal cells, extracellular matrix, and vasculature. b Schematic of the confocal Brillouin imaging setup. L = lens, TL = tube lens, PMT = photomultiplier, P = pinhole, EF = emission filter, DM = dichroic mirror, FC = fiber coupler/collimator, QWP = quarter waveplate, HWP = half waveplate, PBS = polarizing beam splitter, S = shutter, FR = Faraday rotator, CL = cylindrical lens, V = VIPA, Sl = adjustable slit, V = VIPA, I = iris.