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. 2021 Sep 27;4:1133. doi: 10.1038/s42003-021-02662-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Row shows representative maps of Brillouin shift for oocytes from fetal ovaries (E17.5), primordial follicles (P7), secondary follicle (P14) and antral follicle (P21) ovaries. Scale bar = 40 μm. Blue regions within the antral follicle (P21) indicate fluid lumena. b Corresponding Brillouin loss tangent for a. c Boxplot of BLT for oocytes at various stages of follicle development. Each data point corresponds to the average signal of one oocyte. N = 35, 45, 45, 21 for E17.5 germ cells and oocytes from primordial, secondary, and antral follicle stage, respectively. ***P < 0.001.