TABLE 1.

Detection of E. phagocytophila by microscopy, nested PCR, and TaqMan PCR

Day	Cow 1			Cow 2
	% Infected leukocytes	Result of:		% Infected leukocytes	Result of:
		Nested PCR	TaqMan PCR (molecules/μg)		Nested PCR	TaqMan PCR (molecules/μg)
0	0	−	0	0	−	0
4	0	+	1.9 × 102	0	+	2.5 × 103
5	0	+	9.4 × 102	1	+	3.2 × 105
6	5	+	2.5 × 103	11	+	1.5 × 106
7	13.5	+	4.1 × 106	17	+	3.1 × 106
8	18	+	3.3 × 107	20	+	2.1 × 107
9	16.5	+	6.8 × 106	6	+	9.5 × 105
10	7	+	1.1 × 104	3	+	6.8 × 104
11	1	+	3.8 × 103	1	+	4.6 × 104
12	2	+	4.7 × 104	2	+	2.5 × 104
13	2	+	4.5 × 104	1.5	+	1.1 × 104
14	2	+	2.5 × 104	1	+	2.4 × 104
15	1	+	2.4 × 103	0	+	4.0 × 104
16	0	+	2.1 × 103	0	+	8.9 × 103
17	0	+	3.2 × 103	0	+	1.1 × 104
18	0	+	2.4 × 103	0	+	6.5 × 103
19	0	+	2.5 × 102	0	+	7.0 × 103
20	0	+	1.5 × 102	0	+	2.5 × 103
21	0	−	0	0	+	2.9 × 102
22	0	−	0	0	+	6.3 × 102
23	0	−	0	0	−	0