Table 1.

Chromatographic data obtained from the analysis of multi-standards of 18 OCPs injected through GC-ECD that was operated under optimized conditions and obtained ions from the GC-ITD that were used for confirmation, identification, and quality control (limit of detection (LOD) and limit of quantification (LOQ); µg/Kg) of the investigated compounds in fish samples

OCs	Ions (m/z)	tRa	Rb	Tc	LOQ	LOD
α-HCH	183, 219, 111	13.31 ± 1.1	2.3	1.01 ± 0.025	0.056	0.013
γ-HCH	181, 109, 219	14.30 ± 1.2	2.1	1.02 ± 0.021	0.056	0.015
β-HCH	181, 109, 219	15.20 ± 0.9	2.2	1.01 ± 0.025	0.044	0.017
Heptachlor	100, 272, 237	15.37 ± 1.3	2.1	1.02 ± 0.036	0.073	0.019
δ-HCH	109, 183, 219	16.40 ± 1.4	2.2	1.01 ± 0.015	0.055	0.018
Aldrin	66, 79, 263	17.47 ± 1.1	2.2	1.03 ± 0.025	0.042	0.009
Heptachlorepoxide	81, 253, 263	18.61 ± 1.2	2.5	1.02 ± 0.010	0.076	0.019
Endosulfan Ι	241, 195, 339	19.10 ± 1.1	2.4	1.02 ± 0.012	0.044	0.017
p,p-DDE	246, 176, 318	19.8 ± 0.8	2.3	1.04 ± 0.015	0.038	0.009
Dieldrin	79, 263, 277	20.80 ± 1.1	1.5	1.02 ± 0.015	0.062	0.018
Endrin	81, 263, 67	20.70 ± 1.1	1.5	1.03 ± 0.025	0.069	0.018
p,p- DDD	235, 165, 199	21.47 ± 1.3	1.6	1.04 ± 0.015	0.079	0.019
Endosulfan Π	195, 207, 241	21.85 ± 1.1	1.8	1.01 ± 0.021	0.081	0.021
p,p-DDT	235, 199, 165	22.32 ± 1.2	1.5	1.01 ± 0.015	0.068	0.018
Endrin aldehyde	67, 345, 250	22.72 ± 1.1	1.5	1.02 ± 0.012	0.064	0.018
Endosulfan sulfate	387, 272, 237	23.08 ± 1.5	1.5	1.02 ± 0.015	0.078	0.019
Methoxychlor	227, 308, 238	24.50 ± 1.2	2.5	1.01 ± 0.021	0.082	0.023
Endrin ketone	67, 139, 317	25.22 ± 1.1	2.3	1.02 ± 0.026	0.081	0.022

^aReproducibility of tR for each analyte was evaluated during 2 months with minimum of 10 injection of reference standard mixture solution and RSD (%) were determined

^bResolution was determined and the acceptable limit, R not less than 1.5

^ctailing factor (T) was determined according to USP [22] and acceptable limit (T range from 0.9 to 1.1)