Table 6.

VsEP response parameters in diazepam (60 mg/kg) and BPEW vehicle single dose (SD) protocol. VsEP latencies (µs) and amplitudes (µV) at + 6 dB re: 1.0 g/ms as well as thresholds were obtained over a period of 80 min following a 20-min stable baseline. Data were collected from drug-treated (diazepam [n = 10]) or vehicle-treated (BPEW vehicle [n = 6]: 1.4% benzyl alcohol, 38.5% propylene glycol, 12.7% ethyl alcohol and 48% water) mice. Each time period (20 min) was characterized by averaging 4 to 5 consecutive 5-min measurements. All animals were anesthetized with urethane/xylazine, brain temperatures were held at 36.0 ± 0.2 °C and rectal at 37.0 ± 0.2 °C throughout the experiments. Sample size remained constant over entire experiment

VsEP parameters		Groups	Baseline	1–20 min	21–40 min	41–60 min	61–80 min
Latency (µs)	P1	Diazepam	1341 ± 81	1342 ± 91	1352 ± 94	1354 ± 89	1358 ± 87
		BPEW	1278 ± 589	1274 ± 63	1270 ± 62	1270 ± 62	1268 ± 57
	N1	Diazepam	1646 ± 108	1650 ± 109	1665 ± 115	1669 ± 111	1676 ± 109
		BPEW	1570 ± 92	1559 ± 95	1557 ± 95	1561 ± 89	1561 ± 95
	P2	Diazepam	2053 ± 84	2062 ± 88	2103 ± 102	2112 ± 91	2134 ± 86
		BPEW	1947 ± 100	1954 ± 102	1962 ± 106	1958 ± 96	1955 ± 98
Amplitude (µV)	P1-N1	Diazepam	0.91 ± 0.26	0.90 ± 0.21	0.88 ± 0.26	0.89 ± 0.24	0.88 ± 0.25
		BPEW	0.87 ± 0.17	0.88 ± 0.18	0.94 ± 0.21	0.94 ± 0.19	0.92 ± 0.19
	P2-N1	Diazepam	0.83 ± 0.15	0.90 ± 0.22	0.93 ± 0.19	0.92 ± 0.20	0.93 ± 0.23
		BPEW	0.94 ± 0.22	1.00 ± 0.24	1.01 ± 0.29	1.00 ± 0.23	0.96 ± 0.23
Threshold (dB re: 1.0 g/ms)		Diazepam	− 10.8 ± 0.9	− 9.0 ± 2.1	− 8.7 ± 2.1	− 8.7 ± 2.1	− 8.7 ± 1.5
		BPEW	− 11.0 ± 1.2	− 10.5 ± 0.0	− 10.5 ± 1.9	− 10.5 ± 1.9	− 10.0 ± 1.2