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. 2021 Jul-Sep;16(3):377–385. doi: 10.18502/ijpa.v16i3.7090

Fig. 3:

Fig. 3:

A: SDS-PAGE of recombinant protein P1P2P3 : lane 1, BL21 (negative control); lane 2, induced cells for 2 h; lane3, induced cells for 4 h; Lane M, protein size marker (SMOBIO); lane 4, induced cells for 6 h by 1 mM IPTG was separated in 15% SDS-PAGE. B: Western blot analysis of recombinant protein P1P2P3 with anti His-tag antibody. Lane 1 recombinant protein; Lane M, protein size marker (Fermentas); Lane 2, BL21 (negative control). C: Western blot analysis of recombinant purified protein P1P2P3 with anti His-tag antibody. Lane M, protein size marker (Fermentas); Lane 1, BL21 (negative control); Lane 2, recombinant purified protein