Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 14;9:704683. doi: 10.3389/fcell.2021.704683

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Wang, Zhang, Zhou, Chen, Xiang, Fan, Yu, Cai, Wu, Huang, He, Hu, Ding and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CircPPP1CB inhibits proliferation, angiogenesis, migration, and invasion of bladder cancer cells. (A) CircPPP1CB was significantly upregulated in circPPP1CB-overexpressing T24 and EJ cells. (B) CCK-8 assays indicated that CircPPP1CB reduced cell proliferation rate in bladder cancer cells. (C) Colony formation was evaluated by colony formation assay in negative control or circPPPC1B-overexpressing cells. (D) Tube formation assay was conducted in HUVECs with different conditional medium. Scale bars, 400 μm. (E,F) Transwell migration, invasion assay, and cell scratch assay indicated the biological effects of circPPP1CB in T24 and EJ cells. Scale bars, 200 and 400 μm, respectively. (G) EMT markers (fibronectin, N-cadherin, E-cadherin, and vimentin) and MMP2 were detected by western blotting in different T24 and EJ cells. (H) Immunofluorescence analysis of vimentin in circPPP1CB-overexpressing cells and negative control cells. Scale bars, 200 μm. **p < 0.01 vs. control group.