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. 2021 Sep 14;9:704683. doi: 10.3389/fcell.2021.704683

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Copyright © 2021 Wang, Zhang, Zhou, Chen, Xiang, Fan, Yu, Cai, Wu, Huang, He, Hu, Ding and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MiR-1307-3p reverses the biological effects induced by circPPP1CB. (A,B) Cell proliferation and colony formation ability were evaluated in cells under different conditions by CCK-8 assay and colony formation assay, respectively. (C) HUVECs were cultured with conditional media acquired from different bladder cancer cells to determine effects of miR-1307-3p and circPPP1CB on tumor angiogenesis. Scale bars: 400 μm. (D–F) The biological effects of circPPP1CB and miR-1307-3p were determined by transwell assay (scale bars: 200 μm), cell scratch assay (scale bars: 400 μm), and western blotting. (G) Expression levels of vimentin in cells were detected by immunofluorescence assay. Scale bars: 200 μm. **p < 0.01 vs. control group.