Supplementary Figure 7.

A. FoxO inhibitor treatment upregulates the transcription of lysosomal hydrogen ion transporters in Min6 cells, as measured by RNA-seq. B–C Intracellular pH indicator shows increased intracellular acidification after two days of FoxO inhibitor treatment in Min6 cells. B. Representative images of pHrodo live imaging in Min6 cells. Scale bars = 10 μm. C. Quantification of pHrodo live staining signal intensity in Min6 cells pretreated with loperamide/FoxOi for 48h. D. Representative images of lysotracker staining in Min6 cells. Scale bars = 10 μm.