TABLE 3.

Specificity of primer combinations for the identification of Burkholderia spp.

Primer designationa	Target organisms	Size of PCR product (bp) for:
		B. cepacia genomovar			B. multivorans	B. vietnamiensis	B. gladioli	B. mallei	B. pseudomallei	R. pickettii
		I	III	IV
Eub-16-1 + Eub-16-2	Eubacteria	519	519	519	519	519	519	519	519	519
BuRa-16-1 + BuRa-16-2	Burkholderia and Ralstonia spp.	409	409	409	409	409	409	409	409	409
Eub-16-1 + CeMuVi-16-2457	B. cepacia, B. multivorans, and B. vietnamiensis	463	463	463	463	463
Eub-16-1 + Ce-16-21028	B. cepacia	1,015	1,015	1,015
Eub-16-1 + MuVi-16-21028	B. multivorans and B. vietnamiensis				1,015	1,015
ViMaPs-23-1 + CeVi-23-2	B. vietnamiensis					1,055
Eub-16-1 + Gl-16-2457	B. gladioli						463
Eub-16-1 + MaPs-16-2457	B. mallei and B. pseudomallei							463	463
ViMaPs-23-1 + Ma-23-2	B. mallei							526
Eub-16-1 + Pi-16-2461	R. pickettii									467

^aFor sequences see Table 2.