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. 2021 Sep 14;8:747802. doi: 10.3389/fcvm.2021.747802

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Copyright © 2021 Li, Song, Chen, Chen, Zhang, Liu, Li, Dong, Wu and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LncRNA PVT1 knockdown alleviates GSDMD-mediated pyroptosis in myocardial I/R mice. (A) RT-qPCR of the expression of GSDMD mRNA in myocardial tissues of five groups: control, sham, I/R, I/R + sh-NC, and I/R + sh-PVT1 groups. Mice in the I/R + sh-NC and I/R + sh-PVT1 groups were injected by 10 μL sh-NC or sh-PVT1 via Lipofectamine 2000 reagent. After 48 h, myocardial I/R model was constructed. No treatment was done for mice in the control group. The chest of mice in the sham operation group was only opened, but was threaded the corresponding part without ligating the LAD. There were 10 mice in each group. (B,C) Immunohistochemistry of the expression of GSDMD protein in myocardial tissues of above groups. Arrow highlights the positive staining of GSDMD. (D–F) Western blot of (E) GSDMD-FL and (F) GSDMD-N expressions in myocardial tissues of each group. Ns: not significant; ***p < 0.001; ****p < 0.0001.