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. 2021 Sep 14;12:705539. doi: 10.3389/fimmu.2021.705539

Figure 5.

Figure 5

Vaccination with recombinant BoHV-4-A-PPRV-H-ΔTK virus expressing the H protein induces PPRV-specific and neutralizing IgG in sheep. (A) Groups (n = 5/5/4) of sheep were inoculated intramuscularly with PBS (black), control vector (BoHV-4-A-ΔTK) (blue), or BoHV-4-A-PPRV-H-ΔTK (red) at days 0 and 21, and at day 42 (indicated by the arrow), all animals were challenged with virulent PPRV ICV’89. At the indicated time points, the serum samples obtained were analyzed for PPRV-specific IgG by ELISA using ICV’89 PPRV-coated plates. Data are presented as 1/X IgG titer for each animal. *p < 0.05 day 14 PC (post-challenge) vs. day 42 in immunized sheep (paired t-test). ***p < 0.001 in vaccinated (BoHV-4-A-PPRV-H-ΔTK) vs. control (PBS and BoHV-4-A-ΔTK) sheep at the same time point (one-way ANOVA with Fisher LSD post-test). (B, C) Neutralizing antibodies titers against (B) PPRV ICV’89 or (C) PPRV Mor’08 were determined for the same animal groups described in (A) and expressed as the reciprocal of the last dilution of serum that blocked 50% of the virus-specific cytopathic effect in flat bottom 96 plates. (B) *p < 0.05 in vaccinated (BoHV-4-A-PPRV-H-ΔTK) vs. control (PBS and BoHV-4-A-ΔTK) sheep at the same time points, at day 42 (before challenge) and at day 14 PC (one-way ANOVA with Fisher LSD post-test). **p < 0.01 day 14 PC vs. day 21 in immunized sheep and p = 0.051 at day 14 PC vs. day 42 in immunized sheep (paired t-test). (C) *p < 0.05; ***p < 0.001 in vaccinated (BoHV-4-A-PPRV-H-ΔTK) vs. control (PBS and BoHV-4-A-ΔTK) sheep at the same time points, i.e., at day 42 (before challenge) and at day 14 PC (one-way ANOVA with Fisher LSD post-test). *p < 0.05 day 21 vs. day 42; ***p < 0.001 day 21 vs. day 14PC and **p < 0.01 at day 42 vs. day 14PC in immunized sheep (paired t-test).