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. 2021 Sep 14;9:720688. doi: 10.3389/fcell.2021.720688

FIGURE 6.

FIGURE 6

LGK974 treatment suppresses shortened body length and chondrocyte defect caused by of COG4p.G516G expression in zebrafish. (A) Scheme of the LGK974 treatment procedure. (B) Graphs show the measured body length of each group at 4 dpf. The data are presented as mean ± SEM. An unpaired two-tailed t-test was used. ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05; ns, not significant. (C) Ventral view of representative Meckel’s cartilage of control and COG4p.G516G-injected embryos with or without LGK974 treatment at 4 dpf following WGA staining and imaged by a confocal microscope. (D) Graphs show the length-to-width ratio of chondrocytes in Meckel’s cartilage in (C) and two more representative Meckel’s cartilage images of each group. The data are presented as mean ± SEM. One-way ANOVA with Tukey’s multiple comparison tests was applied. ****p < 0.0001; ∗∗p < 0.01; ns, not significant. Experiments were performed in two biological replicates with similar results.